Fig. 1
From: Overexpression of MIG-6 in the cartilage induces an osteoarthritis-like phenotype in mice
Mig-6 mRNA and EGFR protein expression in the articular cartilage of cartilage-specific Mig-6-overexpressing mice. a Levels of Mig-6 mRNA determined by qRT-PCR analysis on P0 epiphysis. Individual data points presented with mean ± SEM (P < 0.05). Data analyzed by two-tailed Student t tests from 11 mice per group. b Immunostaining of phosphorylated epidermal growth factor receptor (pEGFR; Tyr-1173) in the knee joints of 11-week-old control and cMig-6over/over (Mig-6over/overCol2a1-Cre+/−) is decreased in response to increased Mig-6 levels. Frontal sections of mouse articular cartilage incubated without primary antibody, as a negative control, exhibited no staining (d). Cartilage-specific Mig-6 KO mice served as a positive control (e). N = 5 mice/genotyping. MFC, medial femoral condyle; MTP, medial tibial plateau. Scale bar = 100 μm