Fig. 2

CD4⁺ T cell Ezh2 is important for T_FH cell and GC B cell responses in cGVHD. Lupus-like cGVHD was induced in bm12 host mice as in Fig. 1. Single cell splenocyte preparations were stained with markers for a T_FH cells (CD4⁺/CXCR5⁺/PD-1⁺) and b GC B cells (CD19⁺/GL-7⁺/CD95⁺). Percentages of splenic T_FH and GC B cells are shown in A1 and B1, respectively. Student’s t test, **p < 0.01. c Spleen sections (4 μM) were blocked and incubated with antibodies against mouse GL-7 (green), CD45.2 (blue, donor CD4⁺ T cell-specific), and CD1d (red). Images are representative of at least 3 sections from individual mice in each experiment and were evaluated in 2 independent experiments