Methotrexate-loaded multifunctional nanoparticles with near-infrared irradiation for the treatment of rheumatoid arthritis

Table 1 Treatments applied to RA-FLS or CIA mice for comparative study of therapeutic efficacy

Group	Treated content	In vivo		Group	Treated content	In vitro
Group	Treated content	MTX dosage (mg/kg) and administration route^a	NIR light (W/cm²)^b	Group	Treated content	MTX concentration (μM)^c	NIR light (W/cm²)^d
Group 1	Saline	–	–	Group 1	Saline	–	–
Group 2	MTX solution (high dose)	35 × 8 times, IP	–	Group 2	MTX solution (low dose)	0.13	–
Group 3	MTX solution (low dose)	0.2 × 1 time, IV	–	Group 3	MTX solution (high dose)	30	–
Group 4	MTX-unloaded MNPs	–, IV	1.96	Group 4	MTX-unloaded MNPs	–	–
Group 5	MTX-loaded MNPs	0.2, IV	–	Group 5	MTX-unloaded MNPs + NIR	–	0.38
Group 6	MTX-loaded MNPs	0.2, IV	1.96	Group 6	MTX-loaded MNPs	0.13	–
				Group 7	MTX-loaded MNPs + NIR	0.13	0.38

^aTreated volume of MNPs; 30 μl/ml, MNP concentration of 1 mg/ml. ^bThe arthritic limb was exposed to NIR light for 10 min using a laser diode (λ = 808 nm) 24 h post-injection. ^cTreated volume of MNPs; 30 μl/ml, MNP concentration of 1 mg/ml. ^dThe RA-FLS was exposed to NIR light for 10 min using a laser diode (λ = 808 nm) 24 h post-incubation

ISSN: 1478-6362