Fig. 3

Validation of candidate protein biomarkers by MRM-MS and immunofluorescence analysis. a–c Quantification of Coro1A (a), Isg15 (b) and Arhgdib (c) levels in the renal tissues of Sle123 (n = 5) and control mice (n = 5) at 12, 24 and 36 weeks, by targeted MRM-MS. The data are presented as mean (SD). d–f Representative images of immunofluorescence staining for Coro1A (d), Isg15 (e) and Arhgdib (f) in renal tissues of Sle123 and control mice at 24 and 36 weeks. Scale bar = 150 μm. g–i Quantification of Coro1A (g), Isg15 (h) and Arhgdib (i) levels in the renal tissues of independent Sle123 mice cohort and three additional SLE mouse models, by targeted MRM-MS. Different time points were selected for each SLE strain based on age of disease onset. The data are presented as mean (SD) (n = 5 for SLE mouse strains, n = 3 for control mice). Statistical analysis was performed by Student’s t test with equal variances. Asterisks denote statistically significant differences; *p value ≤ 0.05, **p value ≤ 0.01, ***p value ≤ 0.001, ****p value ≤ 0.0001, ns, not significant. W, Weeks