Skip to main content
Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: IgG anti-hinge antibodies against IgG4 F(ab’)2 fragments generated using pepsin are useful diagnostic markers for rheumatoid arthritis: implications of the possible roles of metalloproteinases and IgG subclasses in generating immunogenic hinge epitopes

Fig. 3

Inhibition studies for hinge epitope specificity of IgG AHA. Typical cases were shown in a and b. a IgG AHA from RA patient (S-47) against IgG1 TCZ F(ab’)2pepsin was neither inhibited by IgG2 PAN nor IgG4 NTZ F(ab’)2pepsin, but in a dose-dependent manner by both TCZ-and IFX F(ab’)2pepsin. b IgG AHA from RA patient (S-212) against IgG4 F(ab’)2pepsin was inhibited in a dose-dependent manner by IgG4 F(ab’)2pepsin, followed by IgG1 IFX or TCZ F(ab’)2pepsin, and slightly by IgG2 F(ab’)2pepsin. c Percent inhibitions of IgG AHAs from five RA patients (S-41, S-44, S-45, S-46, S-47) against IgG1 TCZ F(ab’)2pepsin by inhibitors (four different IgG F(ab’)2pepsin) at 1000 μg/mL were 36.8–75.6 for TCZ F(ab’)2pepsin, 33.2–74.5 for IFX F(ab’)2pepsin, − 5.4–23.4 for PAN F(ab’)2pepsin, and − 2.8–37.4 for NTZ F(ab’)2pepsin. d When IgG4 NTZ F(ab’)2pepsin was used as coating antigen and four inhibitors at 1000 μg/mL were used, percent inhibitions of IgG AHAs from RA patients (S-03, S-08, S-44, S-212, S-248) were 46.6–77.5 for TCZ, 34.2–75.8 for IFX, 8.2–33.5 for PAN, and 49.2–77.6 for NTZ

Back to article page