Fig. 6

Transcription factor-target gene regulatory network analysis predicts ETV6, SIRT1, and ATF1 as chondroclast signature transcription factors. Transcription factor (TF)-gene target regulatory network analysis was performed in network cluster 1 using iRegulon plugin in Cytoscape (version 3.7.1). a The iRegulon analysis showed the top transcription binding motifs and their associated transcription factors enriched in the cis-regulatory regions of the twenty-two genes of network cluster 1. b–d Regulatory network analysis representing the predicted transcription factors (as octagonal nodes) of the target genes present in network cluster 1 (as oval nodes) showed ETV6 (b), SIRT1 (c), and ATF2 (d) as signature transcription factors of chondroclasts. The networks were visualized by Cytoscape (version 3.7.1), and transcription factors are significantly enriched at an adjusted P value < 0.05