Fig. 4

Effect of OSM and IL-6+sIL-6R on human skin organoid cultures. Dermal biopsy punches (6 mm) obtained from foreskins were placed onto nitrocellulose membranes and treated with OSM or IL-6+IL-6R for 14 days. Stainings were performed on paraffin sections. a Trichrome staining and IHC staining of PDGFRβ. b Double IHC of PDGFRβ/CD31. Two hundred micrometers for original magnification × 4 and 25 μm for original magnification × 40 images, n = 3. c Effect of OSM and IL-6+sIL-6R on the mRNA levels of profibrotic genes in HDMECs. mRNA levels of profibrotic genes were analyzed by quantitative PCR, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001