Fig. 3
Effects of IL13Rα1 on trail expression in RA FLSs. a RA FLSs (n = 3) were transfected with siIL13Rα1 or siCtrl. Total RNA was extracted and subjected to qRT-PCR for analyzing the changes of trail expression. b The expression of trail at RNA level was detected when RA FLSs (n = 3) was transfected with Lv-IL13Rα1 or Lv-Ctrl. c Phosphorylated and total STAT6 protein were detected in Lv-IL13Rα1 or Lv-Ctrl transfected RA FLSs following Tm or CoCl2 stimulation. d, e RA FLSs (n = 3) harboring Lv-IL13Rα1 or Lv-Ctrl were stimulated with STAT6 inhibitor. Total RNA and protein were subjected to qRT-PCR (d) and Western blot (e) for analyzing trail expression at both RNA and protein levels. With similar treatment in d and e, cell apoptosis (f) and Bax or Bcl protein (g) were detected by Annexin V staining and Western blot. Band intensities are quantified and the Bax/Bcl ratio is summarized into bar charts (right). Data are expressed as the mean of three RA samples ± SD and represent three independent experiments (n = 3). Data in a and b: *p < 0.05 vs the mean ± SD of siCtrl or Lv-Ctrl in the presence of ER stressor. Data in d, f, and g: *p < 0.05 vs the mean ± SD of Lv-IL13Rα1 with vehicle