Skip to main content
Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Intervening upregulated SLC7A5 could mitigate inflammatory mediator by mTOR-P70S6K signal in rheumatoid arthritis synoviocytes

Fig. 6

mTOR-P70S6K-4EBP1 activation and downstream upregulation of MMP13 by Trp in FLS from RA patients. FLS were cultured with or without extra Phe, Trp, and Kyn at the concentration of 1 mM for 4 h and stimulated with IL-1β for 24 h. a The protein expression levels of p-mTOR, p-P70S6K, p-4EBP1, MMP3, and MMP13 in RA FLS were detected by Western blotting (n = 3). mTORC1 activity was assessed by the levels of phosphorylated mTOR (b), P70S6K (c), and 4EBP1 (d), relative to ACTB loading control. The expression of MMP3 (e) and MMP13 (f) was assessed relative to ACTB loading control. g The protein expression levels of p-mTOR, p-P70S6K, p-4EBP1, MMP3, and MMP13 in RA FLS were detected by Western blotting (n = 3). The cells were cultured with or without Phe and Trp in DEME for 24 h and then stimulated with IL-1β for another 8 h. mTORC1 activity was assessed by the levels of phosphorylated mTOR (h), P70S6K (i), and 4EBP1 (j), relative to ACTB loading control. The expression levels of MMP3 (k) and MMP13 (l) were assessed relative to ACTB loading control

Back to article page