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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: PRMT5 inhibition attenuates cartilage degradation by reducing MAPK and NF-κB signaling

Fig. 6

EPZ inhibits IL-1β-induced NF-κB and MAPKs in human chondrocytes. a Human chondrocytes were cultured for 16 h in serum-free medium, pretreated with EPZ (10 μM) for 24 h, then stimulated with IL-1β (10 ng/mL) at the indicated times. GAPDH, p38, JNK, ERK1/2, p-JNK, p-p38, and p-ERK1/2 expression levels were analyzed by western blotting. b Histogram showing the relative protein expression levels of p-JNK, p-p38, and p-ERK1/2 in human chondrocyte. c Chondrocytes were treated as above; the expression levels of GAPDH, p65, IκB, p-IκB, and p-p65 were analyzed by western blotting. d Histogram showing the relative protein expression levels of p-IκB, and p-p65 in human chondrocyte. Data of independent experiments (n = 3) were presented as mean ± SD and analyzed using the one-way analysis of variance with the SNK post hoc test. *P < 0.05 vs. the IL-1β group. #P < 0.05 vs. the con group

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