Fig. 5

Tyro3TK promotes CD14⁺CD16⁻ monocyte-mediated osteoclastogenesis in RA. Purified CD14⁺CD16⁻ monocytes from RA patients (n = 8) were cultured with rhM-CSF (30 ng/ml) and rhRANKL (50 ng/ml) under different conditions for osteoclast differentiation. Seventeen days later, the cells were harvested for TRAP staining. The representative charts and the statistical results were shown. a Different concentrations of rhGas6 (0 ng/ml, 10 ng/ml, 50 ng/ml, and 100 ng/ml) were supplemented for osteoclast differentiation (n = 3 per group, **P = 0.006). b Different concentrations of anti-Tyro3TK antibody (0 ng/ml, 50 ng/ml, 100 ng/ml, and 200 ng/ml), and 50 ng/ml rhGas6 was supplemented for osteoclast differentiation (n = 5 per group, ***P < 0.001). **P < 0.01, ***P < 0.001 (one-way ANOVA test followed by Dunn’s post-test for multiple comparisons, a, b)