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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: MicroRNA-15b shuttled by bone marrow mesenchymal stem cell-derived extracellular vesicles binds to WWP1 and promotes osteogenic differentiation

Fig. 5

Activation of the NF-κB signaling pathway leads to the repression of osteogenic differentiation of BMSCs. BMSCs were treated with PBS, TNF-α, DMSO, or Tanshinone-I. a The expression of IκBα and p65, as well as phosphorylation of IκBα and p65 in BMSCs evaluated by Western blot analysis normalized to GAPDH. b, c ALP activity in BMSCs (× 200). d The expression of Runx2, OPN, and OCN in BMSCs with osteogenic differentiation for 14 days assessed by Western blot analysis normalized to GAPDH. *p < 0.05 vs. BMSCs treated with PBS. #p < 0.05 vs. BMSCs treated with DMSO. e The mineralization of BMSCs determined using ARS staining after treatment with TNF-α, PBS, Tanshinone-I, or DMSO (× 200). *p < 0.05 vs. BMSCs treated with PBS. #p < 0.05 BMSCs treated with DMSO. The measurement data were shown as mean ± standard deviation and compared by independent sample t test. The cell experiment was repeated three times

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