Fig. 2

Immunofluorescence in SS minor salivary gland and human tonsil for detection of LC3B-positive lymphocytes. LC3B staining in SS MSG (a) [the two white arrows indicate LC3B positive epithelial cells (a) and LC3B positive lymphocytes (b)] and human tonsil (c) [LC3B (green); × 25 magnification]. LC3B staining for detection of LC3 puncta in cytoplasm of lymphocytes from MSG (b) and human tonsil (d) [LC3B+ (green) and Hoechst stain; × 60 magnification]. Co-localization in MSG [e × 25 magnification and f × 40 magnification] and tonsil [g × 25 magnification and h × 40 magnification] of autophagy marker LC3B [LC3B+ (green)] with T lymphocytes [CD3+ (red)]. Co-localization in MSG [i × 25 magnification and l × 40 magnification] and tonsil [m × 25 magnification and n × 40 magnification] of autophagy marker LC3B [LC3B+ (green)] with B lymphocytes [CD20+ (blue)]. White squares in a, c, e, g, i, and m indicate the areas that have been magnified [× 60 magnification (b, d) and × 40 magnification (f, h, l, n), respectively]