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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Enhanced BMP-2/BMP-4 ratio in patients with peripheral spondyloarthritis and in cytokine- and stretch-stimulated mouse chondrocytes

Fig. 3

Regulation of Bmp-2 and -4 gene expression in response to cytokine stimulation. a Total RNA was extracted from mouse ankle enthesis after organotypic culture, for real-time PCR analysis. The explants were stimulated 72 h by a cocktail of pro-inflammatory cytokines (TNF-α, IL-22, IL-17, IL-6 and IL-1β, 10 ng/mL each). Gene expression of Bmp-2 and -4 and the Bmp-2/Bmp-4 ratio were compared to the contralateral non-treated samples. b–d Cultured chondrocytes (black bars), osteoblasts (white bars), and tenocytes (gray bars) were treated 24 h by either TNF-α (10 ng/mL), IL-17 (100 ng/mL), or IL-22 (100 ng/mL). Gene expression of Bmp-2 (b) and -4 (c) and the Bmp-2/Bmp-4 ratio (d) were compared to non-treated control cells

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