Fig. 4

Co-culture of fibroblasts and monocytes enhances angiogenic potential, mediated through CD147. Supernatants from co-cultures of HT1080 and MM6 cells derived from the previous experiments were diluted 1:2 in full medium, with or without the neutralizing anti-CD147antibody (2 ng/mL), and incubated for 6 h with the human EaHy926 endothelial cells (8 × 10⁴ cells) which were seeded in wells coated with Coultrex®. a Representative images (×20 magnification) and c quantification of the closed lumen tube-like structures (n = 10). Confluent EaHy926 endothelial cells were scratched and washed, and b representative images obtained at the beginning of the experiment (0 h) and after 24 h (×20 magnification). d The subtraction of the area at T24 from the area at T0, resulting in the area to which endothelial cells migrated to close the wound migration area, was calculated (n = 7)