Table 2 Total number of cells recovered and cell type recovery overall is comparable between preservation methods in frozen media (CryoStor® CS10) compared to fresh media (RPMI) in the 18 cell clusters within the 9 cell groupings
Subject and preservation method | P1 CryoStor | P1 RPMI | P2 CryoStor | P2 RPMI | P3 CryoStor | P3 RPMI | Avg. % cell type per preservation | ||
|---|---|---|---|---|---|---|---|---|---|
Total number cells recovered | 2576 | 3549 | 1707 | 3846 | 1959 | 1274 | CryoStor | RPMI | |
Main cell grouping | Clusters | ||||||||
T/NK cell (%) | 0, 11 | 37 | 19 | 6 | 14 | 15 | 2 | 22 | 14 |
Epithelial/RBC (%) | 1, 4 | 18 | 5 | 19 | 13 | 25 | 40 | 20 | 14 |
Macrophages (%) | 2 | 24 | 15 | 0 | 1 | 0 | 0 | 10 | 7 |
Keratinocytes (%) | 3, 8, 10, 12, 13, 14, 16 | 7 | 48 | 26 | 31 | 22 | 32 | 17 | 38 |
Epithelial (%) | 5 | 1 | 1 | 24 | 10 | 5 | 6 | 8 | 5 |
Fibroblast (%) | 6, 17 | 5 | 6 | 5 | 14 | 7 | 0 | 6 | 9 |
Pericyte (%) | 7, 9 | 6 | 3 | 14 | 13 | 22 | 19 | 13 | 10 |
Dendritic cells (%) | 15 | 2 | 2 | 4 | 3 | 3 | 0 | 2 | 2 |
Lymphatic endothelial (%) | 18 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |