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Table 2 Total number of cells recovered and cell type recovery overall is comparable between preservation methods in frozen media (CryoStor® CS10) compared to fresh media (RPMI) in the 18 cell clusters within the 9 cell groupings

From: Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

Subject and preservation method P1
CryoStor
P1
RPMI
P2
CryoStor
P2
RPMI
P3
CryoStor
P3
RPMI
Avg. % cell type per preservation
Total number cells recovered 2576 3549 1707 3846 1959 1274 CryoStor RPMI
Main cell grouping Clusters         
 T/NK cell (%) 0, 11 37 19 6 14 15 2 22 14
 Epithelial/RBC (%) 1, 4 18 5 19 13 25 40 20 14
 Macrophages (%) 2 24 15 0 1 0 0 10 7
 Keratinocytes (%) 3, 8, 10, 12, 13, 14, 16 7 48 26 31 22 32 17 38
 Epithelial (%) 5 1 1 24 10 5 6 8 5
 Fibroblast (%) 6, 17 5 6 5 14 7 0 6 9
 Pericyte (%) 7, 9 6 3 14 13 22 19 13 10
 Dendritic cells (%) 15 2 2 4 3 3 0 2 2
 Lymphatic endothelial (%) 18 1 1 1 1 1 0 1 1