Fig. 1

Effects of MPA on the survival of human DC subsets. Purified human pDCs and mDCs were incubated for 24 h with different concentrations of MPA (1−10 μM) or vehicle, in the presence of CpG-ODN and R848, respectively. Viable cells were measured by annexin-V staining (a, b). The annexin-V staining profile of one representative experiment out of five independent experiments is shown (a). Percentages of annexin V-negative cells are shown in a and b. One set of experiments was performed on DCs from one donor, and the data are shown as the mean ± SEM of five independent donors. Statistical significance was determined using a paired t test