Skip to main content
Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Mycophenolic acid, the active form of mycophenolate mofetil, interferes with IRF7 nuclear translocation and type I IFN production by plasmacytoid dendritic cells

Fig. 4

Effect of MPA on the nuclear translocation of IRF7 in pDCs. Purified human pDCs were incubated with MPA (1 or 10 μM) or vehicle, followed by the addition of medium alone or CpG-ODN. a After 4 h of culture, IRF7 mRNA in the DC subsets was analyzed by real-time PCR. One set of experiments was performed on DCs from one donor, and the data are shown as the mean ± SEM of three independent donors. b, c After 3 h of culture, the expression of intracellular IRF7 was analyzed by flow cytometry. The staining profile of IRF7 for one representative experiment out of three independent experiments is shown (b). The mean fluorescence intensity (MFI) of IRF7 is shown, and one set of experiments was performed on pDCs from one donor, and the data are shown as the mean ± SEM of three independent donors (c). d After 3 h of culture, pDCs were visualized by immunofluorescence with anti-IRF7 antibody (green) and nuclei staining with DAPI (blue). Similar results were observed in all five independent donors, and representative images are shown. e For measuring the IRF-7 translocation in cell nuclei, stained cells on each slide were counted. Cells were regarded as negative when the IRF7 expression level in the cytoplasm was higher and distinguishable from that in the nucleus. The ratio of nuclear IRF7-negative cells was analyzed based on 50 cells from each donor. One set of experiments was performed on pDCs from one donor, and the data are shown as the mean ± SEM of five independent donors. Statistical significance was determined using a paired t test (*p < 0.05, **p < 0.01)

Back to article page