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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Mycophenolic acid, the active form of mycophenolate mofetil, interferes with IRF7 nuclear translocation and type I IFN production by plasmacytoid dendritic cells

Fig. 5

Effect of MPA on intracellular molecules in DC subsets. Purified human pDCs and mDCs were incubated with MPA (1 or 10 μM) or vehicle, followed by the addition of medium alone, CpG-ODN, or R848. a, b After 90 min of culture, the expression of intracellular pAKT was analyzed by flow cytometry. The staining profile of pAKT for one representative experiment out of three independent experiments is shown (a). The MFI of pAKT is shown and one set of experiments was performed on pDCs from one donor, and the data are shown as the mean ± SEM of three independent donors (b). c After 4 h of culture, STAT4 in the mDC subsets was analyzed by real-time PCR. One set of experiments was performed on DCs from one donor, and the data are shown as the mean ± SEM of three independent donors. Statistical significance was determined using a paired t test (*p < 0.05, **p < 0.01)

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