Fig. 1
ICAM-1 expression and IL-6 and IL-8 secretion in HUVECs stimulated with SSc-ICs, PAPS-ICs, SLE-ICs, or NHS-ICs. Endothelial cells were incubated with SSc-ICs, PAPS-ICs, SLE-ICs, or NHS-ICs (1:2 dilution). IL-1β (50 U/ml) and LPS (1 μg/ml) were used as positive controls. Histograms represent mean + standard error of the mean (SEM). *p < 0.01; **p < 0.001; ***p < 0.0001 versus medium. a ICAM-1 expression (O.D.) [mean ± SD]. IL-1β [429.8 ± 151.9] versus medium [2.75 ± 1.71] (p < 0.0001) versus NHS-ICs (p < 0.0001). LPS [979.5 ± 180.3] versus medium (p < 0.0001) versus NHS-ICs (p < 0.0001). ATA-ICs [134 ± 16.27] versus medium (p = N.S.) and versus NHS-ICs [34.25 ± 17.63] (p = N.S.). ACA-ICs [297.3 ± 153.7] versus medium (p < 0.001) versus NHS-ICs (p < 0.01). ARA-ICs [157.8 ± 17.61] versus medium (p = N.S.) versus NHS-ICs (p = N.S.). Anti-Th/To-ICs [375 ± 42.16] versus medium (p < 0.0001) versus NHS-ICs (p < 0.0001). PAPS-ICs [311.3 ± 151.7] versus medium (p < 0.001) versus NHS-ICs (p < 0.001). SLE-ICs [216 ± 84.75] versus medium (p = N.S.) versus NHS-ICs (p = N.S.). NHS-ICs [34.25 ± 17.63] versus medium (p = N.S.). b IL-6 expression (pg/ml) [mean ± SD]. IL-1β [1414 ± 161.2] versus medium [191 ± 0.70] (p < 0.0001) versus NHS-ICs (p < 0.0001). LPS [2900.5 ± 141.4] versus medium (p < 0.0001) versus NHS-ICs (p < 0.0001). ATA-ICs [584.5 ± 48.79] versus medium (p < 0.01) and versus NHS-ICs [222.5 ± 31.82] (p = N.S.). ACA-ICs [582 ± 15.56] versus medium (p < 0.01) versus NHS-ICs (p = N.S). ARA-ICs [785.5 ± 210] versus medium (p < 0.001) versus NHS-ICs (p < 0.001). Anti-Th/To-ICs [786 ± 90.51] versus medium (p < 0.001) versus NHS-ICs (p < 0.001). PAPS-ICs [504.5 ± 193] versus medium (p = N.S.) versus NHS-ICs (p = N.S.). SLE-ICs [276.5 ± 40.31] versus medium (p = N.S.) versus NHS-ICs (p = N.S.). NHS-ICs [222.5 ± 31.82] versus medium (p = N.S.). c IL-8 expression (pg/ml) [mean ± SD]. IL-1β [1186 ± 101.8] versus medium [1.5 ± 0.6] (p < 0.0001) versus NHS-ICs (p < 0.0001). LPS [1659 ± 178.2] versus medium (p < 0.0001) versus NHS-ICs (p < 0.0001). ATA-ICs [721.5 ± 108.2] versus medium (p < 0.0001) and versus NHS-ICs [222.5 ± 31.82] (p < 0.001). ACA-ICs [814.5 ± 47.38] versus medium (p < 0.0001) versus NHS-ICs (p < 0.0001). ARA-ICs [948 ± 69.3] versus medium (p < 0.0001) versus NHS-ICs (p < 0.0001). Anti-Th/To-ICs [214 ± 22.63] versus medium (p = N.S) versus NHS-ICs (p = N.S.). PAPS-ICs [167 ± 137.2] versus medium (p = N.S.) versus NHS-ICs (p = N.S.). SLE-ICs [434 ± 11.31] versus medium (p < 0.001) versus NHS-ICs (p = N.S.). NHS-ICs [218.2 ± 28.51] versus medium (p = N.S)