Fig. 5

Migration and invasion of rheumatoid arthritis-fibroblast-like synoviocytes (RA-FLSs) promoted by IL-6 and Cyr61 secretion. a, d, e, g Wound-closure over 17 h. b ECIS proliferation analysis over 72 h. c, f Western blotting for Cyr61 protein detection. h, i Cyr61-stimulated invasion of RA-FLSs in transwells over 24 h ± antiCyr61 ab. f, g transfection with 20 pmol/L of small-interfering Cyr61 RNA (siCyr61) or siNC (negative control). IL-6 for 17 h (a, d, g: 200 ng/mL); IL-6 for 2 h (c, f: 20 ng/mL); IL-6 and Cyr61 protein for 72 h (b: IL-6: 200 ng/mL, Cyr61 protein: 100 ng/mL) Cyr61 protein (a, e: 100 ng/mL; I: 800 ng/mL); antiCyr61 antibody (ab) for 2 h (d: 100 ng/mL; e: 50 ng/mL, i: 50, 100, 200 ng/mL) before IL-6 and Cyr61 protein treatment. h Original magnification × 10. Values are means (± standard deviation) of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001