Skip to main content
Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Chebulinic acid is a safe and effective antiangiogenic agent in collagen-induced arthritis in mice

Fig. 6

Effects of chebulinic acid (CI) treatment on vascular endothelial growth factor A (VEGF)-mediated downstream signaling pathways in human synovial microvascular endothelial cells. ac Western-blot analyses show that in comparison to the untreated control, VEGF can induce significant Erk1/2 (~ 42 kD), p38 MAPK (~ 43 kD), and Akt (~ 60 kD) phosphorylation (n = 6, data are expressed as mean ± SEM; VEGF vs control, +P < 0.05, one-way ANOVA). However, CI treatment significantly inhibits VEGF-mediated phosphorylation of Erk1/2, p38, and Akt (n = 6, Data are expressed as mean ± SEM. VEGF+CI vs VEGF, *P < 0.05, one-way ANOVA). The blots were re-probed respectively with total Erk1/2 (~ 42 kD) or p38 MAPK (~ 40 kD) or Akt (~ 60 kD) antibodies. Figures are representative of six separate experiments under similar conditions. d CI treatment shows no significant changes in systolic and diastolic blood pressure in mice (n = 6) when compared to untreated controls (n = 6)

Back to article page