Fig. 6
KPL-404 blocks T cell-induced B cell activation in PBMC cultures from SjS and SLE patients. PBMCs were cultured in the presence of 10 μg/ml IgG4 isotype control or anti-CD40 Abs KPL-404 or G28-5 (16–18 h of cell culture). Cells were left unstimulated (media control) or stimulated with CD3/CD28 cross-linker IC, or F(ab′)2 goat anti-human IgM (anti-IgM) and B cell activation was assessed by the expression of the activation markers CD69 and CD86 on gated live CD19+ B cells. Representative flow cytometry data from one SLE donor is presented on Fig. S5. a, b Cumulative data, depicting the expression of CD69 (a) and CD86 (b) in seven individual SjS donors. c, d Cumulative data, depicting the expression of CD69 (c) and CD86 (d) in eleven individual SLE donors. Data are expressed as fold change MFI over IgG4-teated, media controls. Bars represent mean and standard deviation. Data were analyzed using one-way ANOVA on log-transformed data with matched mixed-effects modeling for multiple comparisons tests of significance between different conditions, *p < 0.05. Only comparisons between IgG4 vs KPL-404 of IC-stimulated samples are shown. Complete statistical analysis of the data is presented in Fig. S5