Fig. 7

Effects of KPL-404 and G28-5 on cytokine production in PBMC cultures from healthy donors and autoimmune patients. PBMCs were stimulated for 18 h. with media control, IC, or anti-IgM in the presence of either IgG4 isotype control, KPL-404, or G28-5 anti-CD40 antibodies. Supernatants analyzed for production of key inflammatory cytokines. Shown are combined results from six HD (a), seven SjS (b), and six SLE donors (c). Additional data is presented in Fig. S7. The data for each sample and each cytokine were normalized against media controls and is expressed as a fold change. Comparison between IgG4 isotype and KPL-404 of unstimulated samples (media control) are shown (all samples), or differences in IFNβ production in response to anti-IgM stimulation (HDs). Statistical analysis were performed using one-way Friedman multiple comparisons test and one-way ANOVA mixed-effects analysis (for HD due to missing values); *p < 0.05