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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: HDAC2 interacts with microRNA-503-5p to regulate SGK1 in osteoarthritis

Fig. 3

Upregulation of miR-503-5p facilitates proliferation and suppresses apoptosis of OA chondrocytes and promotes secretion of inflammatory cytokines. a Successful modeling of OA rats confirmed by Masson staining (50 μm). b Representative images of cartilage tissues of OA rats stained by Masson staining (50 μm). c Expression of miR-503-5p in OA rats measured by RT-qPCR assay. d Apoptosis in OA chondrocytes transfected with overexpressed miR-503-5p examined by flow cytometry. e Gray value analysis of apoptosis-related factors (Caspase-3, Bax, and Bcl2) in OA chondrocytes transfected with overexpressed miR-503-5p. f The protein expression of apoptosis-related factors (Caspase-3, Bax, and Bcl2) in OA chondrocytes transfected with overexpressed miR-503-5p measured by Western blot analysis (n = 10). g Proliferation of primary chondrocytes in OA rats treated with upregulated miR-503-5p detected by MTT assay (n = 10). h The expression of inflammatory cytokines in primary chondrocytes from OA rats treated with upregulated miR-503-5p assessed by ELISA. *p < 0.05. Measurement data were expressed as mean ± standard deviation. Data in compliance with normal distribution and homogeneity of variance between two groups were compared using t test. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. The experiment was repeated three times

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