Fig. 6

HDAC2 suppresses the proliferation of chondrocytes in OA rats through miR-503-5p/SGK1 axis. a Expression of miR-503-5p in primary chondrocytes measured by RT-qPCR assay. b Expression of SGK1 and HDAC2 in primary chondrocytes of HDAC2, normalized to GAPDH, determined by Western blot analysis. c The protein expression of apoptosis-related factors (Caspase-3, Bax, and Bcl2) in OA chondrocytes transfected with overexpressed or under-expressed HDAC2 measured by Western blot analysis. d Apoptosis in OA chondrocytes transfected with overexpressed or under-expressed HDAC2 detected by flow cytometry. e Proliferation of primary chondrocytes in OA rats treated with upregulated or downregulated HDAC2 assessed by MTT assay. f The expression of inflammatory cytokines in primary chondrocytes examined by ELISA. *p < 0.05 vs. normal chondrocytes. Measurement data were expressed as mean ± standard deviation. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. The experiment was repeated three times