Fig. 1

HOTAIR expression in dermal fibroblasts increases Notch-dependent GLI2 transcription. Dermal fibroblasts were transduced with lentiviruses containing a scramble or HOTAIR sequence. RNA and protein were extracted from these cells. a GLI2, H3K27m3 and NID protein levels were assessed by western blot. β-actin was used as a loading control. b Graph represents mean and standard error of densitometry analysis of GLI2 western blot. c GLI2 transcript levels were analysed. Graphs represent the mean and standard error for three independent experiments. RNA and protein were extracted from scramble and HOTAIR-expressing fibroblasts. In addition, scramble and HOTAIR-expressing fibroblasts were treated with the gamma secretase inhibitor R04929097. d GLI2 and NID protein levels were assessed by western blot. β-actin was used as a loading control. GLI2 (e) and HES1 (f) transcript levels were analysed. Graphs represent the mean and standard error for three independent experiments. RNA was extracted from healthy and SSc fibroblasts. In addition, healthy and SSc fibroblasts were treated with the gamma secretase inhibitor R04929097. HES1 (g) and GLI2 (h) transcript levels were analysed. Graphs represent the mean and standard error for three independent experiments. RNA was extracted from healthy and SSc fibroblasts. In addition, healthy and SSc fibroblasts were treated with the gamma secretase inhibitor DAPT. HES1 (i) and GLI2 (j) transcript levels were analysed. Graphs represent the mean and standard error for three independent experiments.*p < 0.05, **p < 0.01, ***p < 0.001