Fig. 8

GLI2 inhibition reduces pro-fibrotic markers in SSc fibroblasts. RNA and protein were extracted from healthy and SSc fibroblasts. In addition, SSc fibroblasts were treated with the Gli inhibitor GANT61. a α-SMA protein levels were assessed by western blot. β-actin was used as a loading control. b Graph represents the mean and standard error for densitometry analysis of the α-SMA western blots. PTCH1 (c), GLI2 (d), COL1A1 (e), COL1A2 (f), α-SMA (g) and CTGF (h) transcript levels were analysed. Graphs represent the mean and standard error for three independent experiments. i Schematic of the HOTAIR/EZH2/miRNA-34a/Notch-mediated activation of GLI2. *p < 0.05, **p < 0.01, ***p < 0.001