Fig. 5

Anti-CD226 antibodies downregulated TIGIT+CD226+ CD4 T cell function. Representative FACS plots showing IFN-γ (a) and TNF-α (b) expression in TIGIT+CD226+ CD4 T cells after stimulation with or without anti-CD226 treatment (left panel). Graphs showing remarkably reduced production of IFN-γ (a) and TNF-α (b) from TIGIT+CD226+ CD4 T cells following CD226 blockade in both patients with DM and HCs (right panel). Comparisons between two groups were carried out using paired two-tailed t tests. Five independent experiments evaluating a total 5 patients with DM and 5 HCs were carried out. *p < 0.05, **p < 0.01, ***p < 0.001