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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Polysaccharide from Angelica sinensis attenuates SNP-induced apoptosis in osteoarthritis chondrocytes by inducing autophagy via the ERK1/2 pathway

Fig. 2

ASP-induced chondrocyte proliferation. a Chondrocytes were treated with or without 200 μg/ml ASP for 6 days. The results are presented as the mean ± SD of three independent experiments (n = 3). b Chondrocytes were collected after 4 days of exposure to 200 μg/ml ASP. Cyclin D1 and p21 were assessed by western blot (quantified in c and d). e After treatment with the culture medium, 200 μg/ml ASP alone or cocultured with the indicated concentration of P276-00 (2.5 μM) for 6 days. g Chondrocyte proliferation rate was detected by Edu assay at day 4 (quantified in f). Data were presented as the mean ± SD of three independent experiments(n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.0001, and statistical significance was determined by Student’s t test

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