Fig. 5

ASP activated the ERK1/2 signaling pathway in chondrocytes stimulated with SNP. Chondrocytes were pretreated with ASP for 4 h then stimulated with SNP (1 mg/ml) for 24 h. a, b. The level of Raf, Ras, p-MEK1/2, and p-ERK1/2 in chondrocytes after stimulation were measured by western blot. b–e. Quantitative analysis of Raf, Ras, p-MEK1/2, and p-ERK1/2 levels. The data are presented as the mean ± SD of three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ^#p < 0.0001, and statistical significance was determined by one-way ANOVA