Skip to main content
Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Proinflammatory T cell polarization is already present in patients with early knee osteoarthritis

Fig. 1

Flow cytometry analysis of CD4+ T cell surface marker expression. Representative flow cytometry histograms of CD4+ T cell surface marker expression are shown. In short, mononuclear cells were isolated from PB, SF, and SM by density gradient centrifugation. CD3+ MACS-isolated T cells from PB, SF, and SM were stained with monoclonal antibodies (mAb) for the following surface markers: CD4, CD25, CD127, CXCR3, CCR5, CCR3, CCR4, CD161, and CCR6. Before flow cytometric detection, 7-AAD was added to the cell suspensions to exclude cell debris and dead cells. The cutoff for all cell surface markers was defined based on fluorescence minus one (FMO) controls (shown as gray overlay population). mAb, monoclonal antibody; MACS, magnetic-activated cell sorting

Back to article page