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Table 6 MACSPlex analysis of cytokine concentration in supernatants of the peripheral blood (PB) and native synovial fluid (SF)

From: Proinflammatory T cell polarization is already present in patients with early knee osteoarthritis

 

PB

SF

p values

n

10

10

 

GM-CSF

0.61 (0.45–1.02)

0.88 (0.40–2.04)

0.655

IFN-γ

18.98 (10.53–33.80)

15.49 (9.38–30.26)

0.715

IL-2

5.2 (2.74–7.69)

5.51 (3.64–7.96)

0.655

IL-4

102.67 (67.65–146.47)

87.37 (20.43–124.88)

0.138

IL-5

2.21 (1.21–4.16)

2.81 (1.32–5.34)

0.715

IL-6

1.18 (0.62–1.84)

19.45 (2.77–71.54)

0.013*

IL-9

46.0 (40.72–54.29)

40.81 (39.57–63.70)

1.000

IL-10

10.79 (8.86–19.85)

8.12 (5.01–15.16)

0.953

IL-12

9.54 (6.05–21.58)

6.96 (4.25–10.51)

0.093

IL-17A

3.34 (1.79–4.02)

3.84 (1.29–4.81)

1.000

TNF-α

5.64 (4.43–7.72)

7.73 (4.20–198.95)

0.249

  1. To evaluate multiple soluble immunomodulating cytokines in PB supernatants and native SF of early OA patients, we utilized the MACSPlex 12 Human Kit (Miltenyi, Germany). All work steps were performed according to the manufacturer’s instruction. Data is shown as median (IQR). Cytokine concentrations are given in pg/ml. Significant differences are marked with an asterisk: *p < 0.05