Fig. 5

TGFBR2 and IGF1R were directly regulated by miR-320a. The construction of luciferase reporter gene system: wild-type (WT) contains part of the 3′UTR of TGFBR1, TGFBR2, and IGF1R with binding sites for miR-21, and the mutant type (MUT) contains a deletion mutation in the predicted miR-320a seeding sequence in the 3′UTR of TGFBR1, TGFBR2, and IGF1R. Increasing the amounts of the miR-320a by transfection of miR-320a mimics represses the luciferase activity of TGFBR2 and IGF1R 3′UTR-WT, but has no influence on the TGFBR1 3′UTR-WT and the mutants. Experiments were repeated at least 3 times. Values are means ± SEM. **p < 0.01 versus scramble controls. ***p < 0.001 versus scramble controls