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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Jo-1 autoantigen-specific B cells are skewed towards distinct functional B cell subsets in anti-synthetase syndrome patients

Fig. 1

Jo-1-specific antibody is detected in sera and stimulated PBMC cultures from subjects with Jo-1 ARS. Serum and PBMCs were collected from individuals with clinical laboratory-confirmed cases of Jo-1 ARS disease (n = 9), IIM patients with “Other Ab” (anti-PL-7, anti-EJ with anti-Ro52, anti-Mi-2, and anti-TIF1- γ), and healthy subjects (n = 8). a ELISA was used to measure serum IgG (left) or IgM (right) antibody bound to purified GST-Jo-1 fusion protein (black) and purified GST protein (gray). b ELISA was used to measure serum IgG (left) or IgM (right) antibody bound to GST-Jo-1 in the absence (black) or presence of excess soluble GST-Jo-1 competitor (gray) to detect Jo-1-specific autoantibody. c, d PBMCs collected from n = 5 Jo-1+ subjects were separated into individual wells and polyclonally stimulated as in the “Methods” section to drive B cell differentiation into antibody-secreting cells. The fraction of positive wells is shown secreting c total antibody or d anti-Jo-1. d Results from two representative patients are shown. Dashed line defines positive wells with a mean fluorescence that is 3 standard deviations above the mean O.D. of all wells

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