Fig. 3

JBCs are enriched among CD21^lo, but not B_ND B cell subsets. PBMCs from healthy controls or Jo-1 ARS patients were stained and analyzed using flow cytometry. Live B cells were identified as in Fig. 2 and a B_ND (IgM^lo IgD⁻ CD27⁻) or b CD21^lo B cells were identified; representative plots are shown for healthy controls (top) or Jo-1 ARS patients (bottom). c–e n = 5 stable Jo-1 ARS (triangles), n = 5 active Jo-1 ARS (circles), and n = 8 healthy controls (diamonds). Only those Jo-1 ARS patients that had > 20 JBC events (n=6) were included for JBC sub-analysis. c The frequency of B_ND B cells among JBCs and non-JBCs in Jo-1 ARS patients (identified as in Fig. 2) or from healthy donors is shown. d, e CD21^lo B cells were further gated on d IgM⁺ or e IgG⁺ cells and the frequencies of the indicated subsets are shown. Individual donors are plotted, and bars represent the mean ± SD. p values were determined using the Mann-Whitney U test, and significant values are indicated on each panel