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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Jo-1 autoantigen-specific B cells are skewed towards distinct functional B cell subsets in anti-synthetase syndrome patients

Fig. 4

JBCs are enriched among CD21lo IgM+ memory cells. PBMCs from healthy controls (top) or Jo-1 ARS (bottom) patients were stained and analyzed based on IgD, IgM, IgG, and CD27 expression using flow cytometry. Live JBCs and non-JBCs (Jo-1 ARS) or total B cells (healthy donors) were identified as in Fig. 2. a, b Representative plots are shown for healthy control (top) or Jo-1 ARS (bottom) donors for the indicated markers. IgM and IgG expression was examined among CD27+ IgD cells gated as in panel a. ch The indicated phenotypic subsets were examined in n = 5 stable Jo-1 ARS (triangles), n = 5 active Jo-1 ARS (circles), and n = 8 healthy controls (diamonds). Only those Jo-1 ARS patients that had > 20 JBC events were included for JBC sub-analysis of either CD21lo (ce) or all cells (fh) for c, f IgD+ CD27+ IgM+, d, g IgD CD27+ IgM+, and e, h IgD CD27+ IgG+ subsets. Individual donors are plotted, and bars represent the mean ± SD. p values were determined using the Mann-Whitney U test, and significant values are indicated on each panel

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