Fig. 6
JBCs are identified following stimulation in vitro but are predominantly IgM+ (non-class-switched). PBMCs from Jo-1 ARS patients with active disease were stimulated as described in the “Methods” section. Cultured cells from 40 to 60 wells per patient were harvested on day 6 and stained for flow cytometry analysis. a Representative pseudocolor plots describing the gating strategy are shown for the selection of live, CD19+ IgM+/− Jo-1 binding populations. The frequencies for each of these plots are indicated. b Cells were gated on live, CD19+ JBCs and the mean frequency of IgM+ and IgM− cells was calculated per donor; individual donors are plotted. c Pseudocolor plot depicting the CD21lo B cells in JBCs and non-JBCs. d The mean frequency of CD21lo B cells within the indicated populations was calculated for per donor and individual donors are plotted. Statistical significance between populations as calculated using the Mann-Whitney U test is indicated. Bars indicate the mean ± SD