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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages

Fig. 4

TNF-α induces abnormal cell morphology of macrophages from HD. Macrophages were cultured with or without diamide (100 nM; for the indicated time; n = 3), IL-6/sIL-6R (10 ng/ml; 24 h; n = 3), and TNF-α (10 ng/ml; 24 h; n = 3) in the presence or absence of nec-1 (20 nM; n = 3) or TOF (300 nM; n = 3). The cells were then stained with specific antibodies against phalloidin (a) or isotype control, PAD4, or 14-3-3η (b), and DAPI (a, b). c 14-3-3η levels were detected by WB. Representative images from three independent experiments are shown. Scale bar, 50 μm

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