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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages

Fig. 5

TNF-α induces cell death of macrophages from HD. a Macrophages were cultured with or without IL-6/sIL-6R (10 ng/ml; n = 3), actinomycin D (1 μg/ml; n = 3), diamide (1 mM, n = 3), or TNF-α (100 ng/ml; n = 3) for 24 h and then analysed by TEM. b WCLs prepared from macrophages cultured with or without TNF-α (100 ng/ml; n = 3) were analysed by IB using specific antibodies against the total or phosphorylation form of MLKL or β-actin. Quantification data for representative images from three independent experiments (n = 3) are shown. Scale bar, 5 μm (upper panel), 2 μm (lower panel)

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