Fig. 4

FICZ increased CD4⁺Foxp3⁺ Tregs in the lungs 1 week after BLM administration. BLM at 0.06 units/animal was intratracheally administered with (denoted as FICZ) or without FICZ (vehicle). a Representative flow cytometry plots of single-cell suspensions extracted from lung tissues stained with anti-mouse CD45, CD3, CD4, Foxp3, and viability dye 1 week after BLM administration are shown. b Summary of total number of CD4⁺Foxp3⁺ Tregs compared between the two groups. n = 5 in each group. *p < 0.05 using Mann-Whitney test