Table 1 Selected genes and their mouse-specific primer sequences
Gene name | Accession number | Sequence | Melt (°C) | Product size |
|---|---|---|---|---|
Gapdh | NM_001289706.1 | F—TGCGACTTCAACAGCAACTC R—CTTGCTCAGTGTCCTTGCTG | 55 | 200 |
Adamts4 | NM_172845.3 | F—TAACTTGAATGGGCAGGGGGGTTC R—AATGGCTTGAGTCAGGACCGAAGG | 60 | 245 |
Adamts5 | NM_011782.2 | F—TCTCCAAAGGTTACGGATGGG R—TCTTCTTCAGGGCTAAGTAGGCAG | 55 | 298 |
Mmp2 | NM_008610 | F—ATTTGGCGGACAGTGACACCAC R—ATCTACTTGCTGGACATCAGGGGG | 59 | 231 |
Mmp3 | NM_010809.1 | F—GCTGAGGACTTTCCAGGTGTTG R—GGTCACTTTTTTGGCATTTGGGTC | 53 | 120 |
Mmp9 | NM_013599 | F—TGGCTTTTGTGACAGGCACTTC R—CGGTGGTGTTCTCCAATGTAAGAG | 55 | 223 |
Mmp13 | NM_008607 | F—GATGACCTGTCTGAGGAAG R—ATCAGACCAGACCTTGAAG | 55 | 357 |
IL1b | NM_008361.4 | F—ACCTGTTCTTTGAAGTTGACGGAC R – TCTTGTTGATGTGCTGCTGCGAG | 55 | 117 |
IL6 | NM_031168.2 | F—CTTCCATCCAGTTGCCTTCTTG R – TGTTGGGAGTGGTATCCTCTGTG | 55 | 104 |
Ccl3 | NM_011337 | F—GAAGGATACAAGCAGCAGCGAG R—GAGAAGAACAGCAAGGGCAGTG | 55 | 115 |