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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Resolvin D1 suppresses pannus formation via decreasing connective tissue growth factor caused by upregulation of miRNA-146a-5p in rheumatoid arthritis

Fig. 6

RvD1 decreased the expression of pro-inflammatory cytokines and CTGF in RA FLS through miRNA-146a-5p. a The cluster heat map shows 24 miRNAs with expression change fold > 1.5 from miRNA microarray data from the RA FLS-treated RvD1 and control (p < 0.05). b The expression of miRNA-146a-5p in RA FLS treated with RvD1 (20, 100 nM) was tested by qRT-PCR. c The expression of miR146a-5p in RA FLS transfected with miR146a-5p mimics and inhibitor was tested by qRT-PCR. d The expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and CTGF the supernatant from RA FLS transfected with miR146a-5p mimics and inhibitor were detected by ELISA. e The expressions of pSTAT3 and STST3 in RA FLS transfected with miR146a-5p mimics and inhibitor by Western blotting. β-actin was used as loading control. Control, RA FLS were treated with PBS; NC, RA FLS were treated with miR146a-5p negative control; mimics, RA FLS were treated with miR146a-5p mimics; iNC, RA FLS were treated with microR146a-5p inhibitor negative control; inhibitor, RA FLS were treated with miR146a-5p inhibitor. All data were represented as the mean ± SD. The differences among three groups were assessed by one-way ANOVA

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