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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Cytotoxic T lymphocyte-associated antigen-4-Ig (CTLA-4-Ig) suppresses Staphylococcus aureus-induced CD80, CD86, and pro-inflammatory cytokine expression in human B cells

Fig. 1

CTLA-4-Ig binds human memory and activated B cells. a CTLA-4-Ig binds peripheral blood memory B cells. Human peripheral blood mononuclear cells (PBMCs) were stained with 10 μg/ml biotinylated-CTLA-4-Ig or control-protein (Ctrl-Ig), followed by streptavidin-PE together with anti-CD20 and anti-CD27 antibodies and analyzed using flow cytometry. Left histograms, one representative binding of CTLA-4-Ig gated on CD20+CD27 naïve B cells (bottom) or on CD20+CD27+ memory B cells (top) is shown (n = 3). Gray peaks, Ctrl-Ig; blue line, CTLA-4-Ig. Middle and right histograms, anti-CD80 or anti-CD86 antibody was used to examine CD80 and CD86 expression on naïve and memory B cells. Gray peak, isotype control; blue line, anti-CD80 or anti-CD86 antibody. b CD19+ B cells were purified from the blood of healthy donors and the purity was determined by anti-CD20 antibody staining. On representative result is shown. c CTLA-4-Ig binds activated B cells. Purified CD19+ B cells from blood were stimulated with anti-IgM and anti-CD40 antibodies for 3 days and the levels of CTLA-4-Ig binding (left), CD80 (middle), and CD86 (right) were examined as described in a. Gray peaks, Ctrl-Ig (10 μg/ml) or isotype control; blue line, CTLA-4-Ig (10 μg/ml) or anti-CD80/86 antibody. Data are representative of three independent experiments

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