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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Cytotoxic T lymphocyte-associated antigen-4-Ig (CTLA-4-Ig) suppresses Staphylococcus aureus-induced CD80, CD86, and pro-inflammatory cytokine expression in human B cells

Fig. 3

CTLA-4-Ig suppresses SAC-induced TNF-α and IL-6 production in B cell. a Purified B cells were stimulated with SAC or anti-IgM plus anti-CD40 antibodies in the presence of CTLA-4-Ig (100 μg/ml) or Ctrl-Ig (100 μg/ml) for 8 h (TNFA) or 48 h (IL6 and LTA), and gene expression was measured as described in Fig. 2b. b Concentration of TNF-α or IL-6 in the supernatant of the SAC-stimulated B cells was analyzed by ELISA. c CTLA-4-Ig suppressed proinflammatory cytokine production by naïve and memory B cells. Sorted naïve and memory B cells were stimulated with SAC in the presence of CTLA-4-Ig or Ctrl-Ig for 8 h (TNFα) or 48 h (IL-6), and the supernatant was collected for ELISA (n = 6). The readout for the Ctrl-Ig treatment results was considered 100% for the calculation of the relative level in each donor

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