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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Cytotoxic T lymphocyte-associated antigen-4-Ig (CTLA-4-Ig) suppresses Staphylococcus aureus-induced CD80, CD86, and pro-inflammatory cytokine expression in human B cells

Fig. 4

CTLA-4-Ig has no effect on SAC- and TD-induced proliferation of human B cells. a CTLA-4-Ig did not affect T-dependent or SAC-induced B cell proliferation. Fifty thousand purified B cells per well were seeded in a 96-well plate and stimulated with SAC or anti-IgM (5 μg/ml) plus anti-CD40 (1 μg/ml) antibodies in the presence of CTLA-4-Ig (100 μg/ml) or Ctrl-Ig (100 μg/ml) for 3 days, and then 1 μCi [3H]-thymidine was added to each well and incubated for an additional day before being harvested (n = 7). The readout of the medium only group was used to calculate the incorporation (fold) of [3H]-thymidine for each donor sample. The bar graph shows the cumulative incorporation (fold) of the samples from 7 donors. b CTLA-4-Ig had no effect on naïve or memory B cell proliferation. Sorted naïve and memory B cells were stimulated with SAC in the presence of CTLA-4-Ig or Ctrl-Ig, and [3H]-thymidine uptake was measured. The bar graph shows the cumulative incorporation in the samples of 3 donors

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