Fig. 5

Expression of Ern1 in macrophages was mediated by exosomal miR-124-3p derived from BMMSCs. a Macrophages ingesting exosomes observed by confocal fluorescence microscope (× 200). b Expression of miR-124-3p in BMMSCs and exosomes detected by RT-qPCR; *p < 0.05 versus mimic NC; #p < 0.05 versus inhibitor NC. c Exosome secretion observed by transmission electron microscope (× 50,000). d The fluorescence PKH67 (Green) after GW4869 treatment observed by confocal fluorescence microscope. e Expression of miR-124-3p and Ern1 in co-cultured macrophages detected by RT-qPCR. f Expression of miR-124-3p and Ern1 in co-cultured macrophages determined by western blot analysis. In d and e, *p < 0.05 versus control; #p < 0.05 versus NC of BMMSCs mimic; &p < 0.05 versus NC of BMMSCs inhibitor. The data in figures were measurement data and described as mean ± standard deviation. Comparison between two groups was performed using unpaired t-test. Multiple-group comparison was made using one-way ANOVA, followed by a Tukey’s post hoc test. The cell experiment was repeated three times