Skip to main content
Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: A bioavailable form of curcumin, in combination with vitamin-D- and omega-3-enriched diet, modifies disease onset and outcomes in a murine model of collagen-induced arthritis

Fig. 3

CUR alone, or in combination with a VO-enriched diet, mitigated the abundance of MMPs in the joints of CIA mice. Joint tissue lysates (20 μg protein each) were resolved on NuPage 4–12% Bis-Tris protein gels and probed in immunoblots with antibodies for mouse MMP-1, MMP-3, MMP-9, and MMP-13. Antibody to mouse GAPDH was used to assess protein loading. Densitometry for band intensity was determined using the AmershamTM Imager 680 analysis software version 2.0. Relative band intensity was determined by normalizing to the GAPDH band intensity for each sample. Graphs represent the relative fold change compared to saline-treated mice normalized to 1. GraphPad Prism 7.05 software was used for statistical analyses. Kruskal–Wallis one-way analysis of variance (ANOVA) followed by Dunn’s multiple comparison post hoc test was used to determine the p values (*p ≤ 0.05)

Back to article page