Fig. 2
From: Functional analysis of novel A20 variants in patients with atypical inflammatory diseases
NF-κB reporter gene activity of TNFAIP3 variants and the expression of A20. a–c NF-κB reporter gene analysis measuring activity of TNFAIP3 variants using A20-deficient HEK293 cells. NF-κB reporter gene activity was induced by a stimulation with 20 ng/mL TNF-α, b co-transfection with MYD88 mutant (L265P), and c co-transfection with CARD11 mutant (F130V). The variant E192K could not suppress the NF-κB reporter gene activity elevated by TNF-α stimulation or co-transfection of GoF mutants of MYD88 or CARD11. d Immunoblot analysis of A20 variants. The expression levels of the variant I310T and known mutants, C243Y and T647P, were reduced