Fig. 5From: Functional analysis of novel A20 variants in patients with atypical inflammatory diseasesImmunoblot analysis to evaluate the deubiquitinase function of TNFAIP3 variants. A20 WT and variants’ constructs were co-transfected into A20-deficient HEK293 cells with plasmids expressing K63-linked ubiquitin and FLAG-TRAF6, which is the A20 ubiquitination target. Deubiquitinase functions of the variants E192K, I310T, and Q709R did not differ from that of the WTBack to article page